Zeta potential changing nanoemulsions based on a simple zwitterion

Simple zwitterions used as auxiliary agents might have the potential to change zeta of oil-in-water (o/w) nanoemulsions on mucosa. The zwitterion phosphorylated tyramine (p-Tyr) was synthesized by phosphorylation Boc-tyramine (Boc-Tyr) using phosphoryl chloride (POCl3). It incorporated with 2% (m/v) in a self-emulsifying lipophilic phase comprising Captex 35, Cremophor EL, Capmul MCM and glycerol 85 at ratio 30:30:30:10 v/v. Phosphate release resulting were evaluated incubating p-Tyr containing nanoemulsion isolated intestinal alkaline phosphatase (AP). Mucus permeating behavior across mucus obtained from porcine small Subsequently, cellular uptake studies accomplished Caco-2 cells. loaded exhibited mean droplet size 43 ± 1.7 nm −8.40 mV. moieties rapidly cleaved after incubation AP shift mV +1.2 revealed significantly (p ≤ 0.001) improved permeation compared same having been pre-treated AP. Cellular changing oily droplets 2.4-fold improved. Phosphorylated seem be an alternative cationic surfactants considered promising for nanoemulsions.